KMID : 0545120070170081390
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Journal of Microbiology and Biotechnology 2007 Volume.17 No. 8 p.1390 ~ p.1393
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Analysis of the Stress Effects of Endocrine Disrupting Chemicals (EDCs) on Escherichia coli
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Kim Yeon-Seok
Min Ji-Ho Hong Han-Na Park Ji-Hyun Park Kyeong-Seo Gu Man-Bock
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Abstract
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In this study, three of the representative EDCs, 17¥â- estradiol, bisphenol A, and styrene, were employed to find their mode of toxic actions in E. coli. To accomplish this, four different stress response genes, recA, katG, fabA, and grpE genes, were used as a representative for DNA, oxidative, membrane, or protein damage, respectively. The expression levels of these four genes were quantified using a real-time RT-PCR after challenge with three different EDCs individually. Bisphenol A and styrene caused high-level expression of recA and katG genes, respectively, whereas 17¥â-estradiol made no significant changes in expression of any of those genes. These results lead to the classification of the mode of toxic actions of EDCs on E. coli
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KEYWORD
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Stress effect, endocrine disrupting chemicals (EDCs), real-time RT-PCR, stress-specific responsive gene, gene expression analysis
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